Black Pearl King Oyster Liquid Culture Syringe

• Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.
• Incubate Petri dishes at 20-25°C (68-77°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 20-25°C (68-77°F) until fully colonized.

• Pleurotus ostreatus can be grown on a variety of lignocellulosic materials, including straw, sawdust, coffee grounds, and agricultural waste.
• The substrate should be pasteurized or sterilized to eliminate contaminants and provide a nutrient-rich environment for the fungus.

• Optimal temperature for mycelium growth: 20-25°C (68-77°F)
• Optimal temperature for fruiting: 15-20°C (59-68°F)

• Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

Pleurotus ostreatus requires light for fruiting. Indirect sunlight or artificial light providing around 1000-2000 lux is sufficient.

Proper air exchange is necessary to provide fresh air and to prevent the buildup of carbon dioxide, which can inhibit the growth of the fungus. Good ventilation also helps in maintaining appropriate humidity and temperature conditions.

The growth cycle of Pleurotus ostreatus from inoculation to harvest can take approximately 4 to 6 weeks. This includes the time for the mycelium to colonize the substrate and the subsequent development of fruiting bodies.

For cultivating Pleurotus ostreatus, the ideal pH range of the substrate is typically between 6.0 and 7.5. Maintaining this pH range helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently.