Brown Beech Liquid Culture Syringe

• Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.
• Incubate Petri dishes at 22-25°C (72-77°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 22-25°C (72-77°F) until fully colonized.

• Hypsizygus tessellatus thrives on a variety of hardwood substrates, including sawdust, wood chips, and supplemented hardwood pellets.
• Ensure the substrate is properly sterilized to avoid contamination and to provide a nutrient-rich environment for the fungus.

• Optimal temperature for mycelium growth: 22-25°C (72-77°F)
• Optimal temperature for fruiting: 10-15°C (50-59°F)

• Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

Low to moderate light conditions are beneficial during the fruiting stage to help the mushrooms orient themselves. Indirect sunlight or artificial light that simulates natural light can be used.

• Proper air exchange is necessary to provide fresh air and to prevent the buildup of carbon dioxide, which can inhibit the growth of the fungus.
• Good ventilation helps maintain appropriate humidity and temperature conditions.

• Colonization of the substrate typically takes 3-4 weeks, depending on environmental conditions.
• Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.
• Total growing time from inoculation to harvest is approximately 6-8 weeks.

For cultivating Hypsizygus tessellatus, the ideal pH range of the substrate is typically between 5.5 and 7.0. Maintaining this pH range helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently. Adjusting the pH of your substrate according to these guidelines can significantly impact the success of your cultivation.