Nebrodi or White Elf Liquid Culture Syringe

• Use potato dextrose agar (PDA) or malt extract agar (MEA) for initial culturing of the mycelium.
• Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.

• Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.
• Sterilize the grains and inoculate with the colonized agar from the Petri dishes.
• Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.

Pleurotus nebrodensis can be grown on a variety of substrates including straw, sawdust, and agricultural waste. A commonly used substrate mixture includes pasteurized straw or hardwood sawdust supplemented with bran or other nitrogen-rich additives.

• Optimal temperature for mycelium growth: 20-24°C (68-75°F)
• Optimal temperature for fruiting: 15-20°C (59-68°F)

• Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.
• Mist the substrate regularly to maintain adequate moisture levels.

Nebrodi mushrooms require some light for proper development of fruiting bodies. Indirect sunlight or artificial light with a moderate intensity is sufficient.

• Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.
• Ensure proper ventilation to maintain fresh air exchange.

• Colonization of the substrate typically takes 2-4 weeks, depending on environmental conditions.
• Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.
• Total growing time from inoculation to harvest is approximately 6-8 weeks.

The ideal pH range of the substrate for Pleurotus nebrodensis is typically between 6.0 and 7.0, with a neutral to slightly acidic environment being most conducive to growth. Maintaining this pH range helps optimize the conditions for the mycelium to thrive and produce fruiting bodies efficiently.