Agaricus bisporus, commonly known as the white button mushroom, is one of the most widely cultivated mushrooms globally. It is prized for its mild flavor and versatility in culinary applications. Agaricus bisporus also includes the cremini (brown button) and portobello mushrooms, which are simply different maturity stages of the same species. This mushroom is rich in essential nutrients, including protein, vitamins, and minerals, making it a popular choice for both home cooks and professional chefs.

Our White Button Liquid Culture Syringe contains a pure, carefully isolated sample of mycelium nurtured and expanded in our sterile laboratory. This 10 mL/cc syringe is ideal for inoculating substrates with precision and ensuring rapid colonization. Each syringe is guaranteed to be contaminant-free, ensuring a high success rate for your cultivation projects. We offer a free replacement if needed.

White Button Liquid Culture Syringe

Name: White Button Liquid Culture Syringe
Brand: Mission Myco
SKU: MM-WB-LC-0001-10mL

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SKU: MM-WB-LC-0001-10mL

$20.00

$15.00

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Classification:

Kingdom: Fungi

Phylum: Basidiomycota

Class: Agaricomycetes

Order: Agaricales

Family: Agaricaceae

Genus: Agaricus

Species: A. bisporus

Other Names:

White Button Mushroom

Cremini Mushroom (when immature)

Portobello Mushroom (when mature)

Common Mushroom

Champignon
Use malt extract agar (MEA) or potato dextrose agar (PDA) for initial culturing of the mycelium.

Incubate Petri dishes at 24-27°C (75-80°F) in a dark environment until fully colonized.
Grain spawn (oats, rye, millet, or sorghum) is recommended for inoculating the bulk substrate.

Sterilize the grains and inoculate with the colonized agar from the Petri dishes.

Incubate the grain spawn at 24-27°C (75-80°F) until fully colonized.
Agaricus bisporus thrives on composted organic matter, particularly a mixture of horse manure and straw, supplemented with gypsum. The substrate should be well-aged and properly composted to support vigorous mycelium growth.

Ensure the substrate is pasteurized to eliminate potential contaminants.
Optimal temperature for mycelium growth: 24-27°C (75-80°F)

Optimal temperature for fruiting: 12-18°C (54-65°F)
Maintain high humidity levels of 85-95% during both mycelium growth and fruiting stages.

Mist the substrate regularly to maintain adequate moisture levels, but avoid waterlogging.
Agaricus bisporus does not require light for mycelium growth.

Fruiting bodies can develop in low to moderate light conditions. Indirect light is sufficient to stimulate the formation of mushrooms.
Good air circulation is essential to prevent the buildup of CO2 and to provide fresh air, which is critical during the fruiting stage.

Ensure proper ventilation to maintain fresh air exchange and to help regulate humidity and temperature.
Colonization of the substrate typically takes 2-4 weeks, depending on environmental conditions.

Fruiting can be expected within 1-2 weeks after the substrate is fully colonized and conditions are adjusted for fruiting.

Total growing time from inoculation to harvest is approximately 6-8 weeks.